Let's talk about the comparison of different classifications of PCR machines
Comparison of PCR Instruments for Different Categories
(1) The ordinary basic PCR instrument consists of a host, a heating module, a PCR tube sample base, a hot cover, and control software.
(2) In addition to the structure of a regular PCR instrument, the gradient PCR instrument also has different gradient modules that can adjust parameters such as gradient temperature and gradient time. Therefore, different annealing temperatures and times can be set for different samples in one experiment, which can optimize the PCR experimental conditions in a short period of time and increase the efficiency of PCR research.
(3) The real-time fluorescence quantitative PCR instrument adds a fluorescence signal acquisition system and a computer analysis and processing system on the basis of the ordinary PCR instrument. The fluorescence detection system mainly includes an excitation light source and a detector. The excitation light sources include halogen tungsten lamp light source, argon ion laser, and light-emitting diode LED light source. The former can be equipped with multi-color filters to achieve different excitation wavelengths, while monochromatic light-emitting diode LED has low price, low energy consumption, and long lifespan. However, because it is monochromatic, different LEDs are needed to achieve different excitation wavelengths. The monitoring system includes an ultra-low temperature CCD imaging system and a PMT photomultiplier tube. The former can image multiple points at once, while the latter has high sensitivity but can only scan one sample at a time. It requires scanning one sample at a time to achieve multi sample detection, which takes a long time for a large number of samples.
(4) Compared with ordinary PCR machines, in situ PCR machines use glass slides instead of PCR tubes, and the reaction process is carried out on the surface of the glass slide.
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