Most foreign test kits are designed to block ELISA, such as those from Jinnuo Korea and Yingina Spain, as well as detection kits from Edexcel in the United States and LSI in France. The biggest advantage of blocking ELISAzu is its accuracy, resistance to interference, and low background value. Most of these boxes are coated with swine fever E2 protein, which targets monoclonal epitopes. Generally, due to individual differences, the host's antibody titers against a single epitope can vary greatly, so in some cases, their values may differ.
Most of the reagent kits in mainland China are indirect ELISA, which contain a segment of antigen and multiple epitopes. Therefore, sometimes it is necessary to compare. If the strain mutates at a single epitope that blocks ELISA, the result may be negative.
There is an experimental data from the epidemic prevention and control point: Edexcel is negative, LSI is positive.
Question here: What are the effects of different manufacturers' swine fever vaccines? The infection level of each rabbit varies greatly, is it better to have a higher infection level?
Answer: This is very sensitive and varies depending on the situation, so it should be moderate; Both too low and too high are greatly affected by interference from maternal antibodies. Because the maternal antibodies against swine fever have neutralizing activity, if piglets are in the peak period of maternal antibodies, they will be neutralized, which not only does not have a positive effect, but also consumes maternal antibodies.
Edexcel is coated with E2 protein, while LSI is coated with whole virus, so usually Edexcel's results do not look so good, it is a matter of how to choose between specificity and sensitivity.
E2 protein is the main protective protein induced by swine fever virus to produce neutralizing antibodies in the body, and the antibodies detected by Edwards are not correlated with neutralizing antibodies.
The rabbit body infection rate is an inaccurate method for calibrating antigens. A customer injected a dose of swine fever vaccine into a rabbit without causing any fever, and believed that the swine fever vaccine was not qualified. But using wo fluorescence quantitative PCR detection, the antigen is qualified.
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